Pediatrics Hematology/Oncology - Project Scientist series - Nowicki Lab | University of California Los Angeles - Military Veterans

Pediatrics Hematology/Oncology - Project Scientist series - Nowicki Lab University of California Los Angeles Requisition Number: JPF10120 The Division of Hematology-Oncology in the Department of Pediatrics of the David Geffen School of Medicine at UCLA is seeking a Project Scientist. The appointment will be in the Project Scientist series; academic rank will be commensurate with experience. This is a Non-Senate position. Qualifications: PhD, MD/PhD Prior experience Duties and Responsibilities: Manufacturing products for clinical research according to Good Manufacturing Practice (GMP) guidelines Operate in a GMP regulated environment according current standard operating protocols Meticulous record keeping and maintenance of GMP, GTP and basic research related records and documents. Attend and arrange seminars, training sessions and workshops to maintain competency in evolving current GMP/GTP regulations Performing post-manufacturing lot-release tests: Elisa, Colony-forming Unit (CFU) assay, ELISA, Endotoxin assay, Mycoplasma assay, vector copy number (VCN) test by ddPCR Culture of hematopoietic progenitor cells for in vitro differentiation into T-cells for proof of principle testing of gene delivery Conduct experiments for FDA IND application(s) Conduct DNA, RNA and protein isolation and purification according current GLP research grade protocols Quantification of nucleic acids and proteins Perform PCR and qPCR Perform multiparametric flow cytometry to assist on-going specific NYESO-TCR research project for intracellular, surface and peptide target markers. Culture adhered and non-adherent mammalian cells, preparing media, aliquots and other compounds utilized in tissue culture with proper sterile aseptic technique. Ability to set up co-cultures for killing-assays or peptide release production-analysis Ability to set up experiments utilizing real time in vitro cell imaging system Incucyte Perform ELISA experiments in either cellular supernatants or cellular lysates normalizing results against total protein content Plasmid preparation, bacteria growth on competent E. coli or Stable 3 systems, positive transformants selection and sequences analysis Ability of align sequences on APE-software (or-equivalent) and design plasmids for plasmid-vector construction and PCR analysis Perform plasmid sequencing and re-sequencing Production of high-titer viral vectors, purification and concentration of viral supernatants for retrovirus Production of high-titer viral vectors, purification and concentration of viral supernatants for lentivirus Determination of viral titer by ddPCR on fresh and frozen supernatants Document results of experiments and elaboration of reports for NYESO-TCR team leader Perform experiments associated to survival tittering and transduction efficiency on target cells Design and re-design of viral vectors for large scale manufacture of GMP and GMP-comparable clinical grade virus. Performs other job-related duties as assigned *Interested candidates can apply online at: https://recruit.apo.ucla.edu/JPF10120 The posted UC salary scales * https://www.ucop.edu/academic-personnel-programs/_files/2024-25/july-2024-scales/t37-b.pdf . set the minimum pay determined by rank and step at appointment. The salary range for this position is $90,100 - $111,400. Cultural North Star: The shared values of the DGSOM are expressed in the Cultural North Star, which was developed by members of our community and affirms our unswerving commitment to doing what's right, making things better, and being kind. These are the standards to which we hold ourselves, and one another. Please read more about this important DGSOM program at https://medschool.ucla.edu/cultural-north-star . Anti-Racism Roadmap: The David Geffen School of Medicine Anti-racism roadmap is our co-created path to ensuring racial justice, equity, diversity and inclusion. We are committed to actively dismantling structural racism in our organization as described in more detail at: https://medschool.ucla.edu/diversity-anti-racism-roadmap . UC Regents Statement on Ethical Values and Standards of Conduct: All aspects of searches are confidential and all candidates are expected to review and abide by UC Regents Policy 1111 on Statement on Ethical Values and Standards of Conduct https://regents.universityofcalifornia.edu/governance/policies/1111.html To apply, please visit: https://recruit.apo.ucla.edu/JPF10120 The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy, see: UC Nondiscrimination & Affirmative Action Policy, https://policy.ucop.edu/doc/4000376/DiscHarassAffirmAction jeid-039caed9a646764d836b978c9f6fa0e3