Research Associate II - Human Microbiome Research Institute | Cedars-Sinai Medical Center - Military Veterans

Job Description Cedars-Sinai has established a new Human Microbiome Research Institute that will support investigators studying how the microbiome plays a role in human health and disease with key focus areas in Cancer, Metabolism, Early Life, GI & Liver, and Autoimmunity. The Human Microbiome Research Institute (HMRI) is an innovation hub and coordinating center for Microbiome Research at Cedars-Sinai. The HMRI facilitates exciting human and animal studies from Cedars-Sinai investigators and external collaborators across multiple areas of research. The applicant will have exposure to these broad areas of study with the central uniting theme being that we wish to understand the role of the microbes in and on our bodies in the progression of these diseases. To learn more, please visit?? Human Microbiome Research Institute | Cedars-Sinai. Are you ready to be a part of breakthrough research? The Research Associate II (RA II) will be responsible for carrying out molecular, cellular and live animal techniques for external projects submitted to the HMRI including anaerobic bacterial cultivation, microbial DNA/RNA extraction, library prep for 16S and metagenomic sequencing, bacterial genetics, and gnotobiotic animal experiments. As such, the candidate should have a strong background in microbiology and/or microbiome science. The RA II will also perform PCR and qRT-PCR, ELISAs, perform DNA and RNA extractions, and may learn and take on additional skills such as flow cytometry, FACS sorting, sample preparation for single-cell and single-nuclei sequencing, and advanced microscopy techniques. #Jobs Primary Job Duties and Responsibilities: Performs a variety of increasingly sophisticated Microbiome experimental protocols and procedures and assist in the design of new protocols and techniques. Keeps accurate and detailed records of experiments and results, and assist in identifying and troubleshooting unexpected results. Maintains lab equipment and related records and may coordinate the use of lab equipment. Assists in the operation of specialized equipment, as required by the area of research. Orients research laboratory assistants or lower-level research associates and undergraduate student researchers on day-to-day lab operations and routine procedures. Department-Specific Responsibilities: Responsible to carry out Microbiome-specific molecular, cellular, and live animal techniques for external projects submitted to the HMRI. Cultivate live bacteria from human and animal tissues, create bacterial stocks, and revive bacteria as needed. Maintain the bacterial culture collection and conduct in-vitro co-culture experiments. Perform animal experiments in gnotobiotic mice, including gavage of bacteria and bacterial communities. Work with highly sterile technique, with exquisite attention to detail in the extraction of DNA for 16S and metagenomics. Carry out sequencing library preps Conduct PCR and qRT-PCR assays. Perform ELISAs. Learn and acquire additional skills such as flow cytometry, FACS sorting, sample preparation for single-cell and single-nuclei sequencing, and advanced microscopy. Qualifications Education: Bachelor's Degree in a Science related field is required. Experience and Skills: At least one (1) year of research laboratory experience in microbiology or microbiome-related sciences is required. Three (3) years is highly desired. Excellent organization and problem-solving skills, detail-oriented, and self-motivated with the ability to multitask. Ability to work closely and effectively within a team environment, with the ability to work well independently on specified projects. Strong interpersonal skills for interaction with peers and other scientists in the research team as well as with the administrative team within the Center. About Us Cedars-Sinai is a leader in providing high-quality healthcare encompassing primary care, specialized medicine and research. Since 1902, Cedars-Sinai has evolved to meet the needs of one of the most diverse regions in the nation, setting standards in quality and innovative patient care, research, teaching and community service. Today, Cedars- Sinai is known for its national leadership in transforming healthcare for the benefit of patients. Cedars-Sinai impacts the future of healthcare by developing new approaches to treatment and educating tomorrow's health professionals. Additionally, Cedars-Sinai demonstrates a commitment to the community through programs that improve the health of its most vulnerable residents. About the Team Cedars-Sinai is one of the largest nonprofit academic medical centers in the U.S., with 886 licensed beds, 2,100 physicians, 2,800 nurses and thousands of other healthcare professionals and staff. Choose this if you want to work in a fast-paced environment that offers the highest level of care to people in the Los Angeles that need our care the most. Req ID : HRC1307217 Working Title : Research Associate II - Human Microbiome Research Institute Department : Research - Digestive and Liver Diseases Business Entity : Cedars-Sinai Medical Center Job Category : Academic / Research Job Specialty : Research Studies/ Clin Trial Overtime Status : NONEXEMPT Primary Shift : Day Shift Duration : 8 hour Base Pay : $21.26 - $36.14