Staff Associate I | Columbia University - Military Veterans

The department of Neurology is currently seeking a Staff Associate I. Wet-lab Work ATAC-seq protocol optimization and project -Responsible for shipping and receivingROSMAP(500) samples related toATAC-seq project -Nuclei processing and sorting of all samples -PCRamplification and library preparation of all samples -downstream quality control steps for all samples -Library sample pooling and shipping for all samples -Extensive record keeping throughout each stage: estimated project budget, inventory, manifest log, progress log, quality control data, etc. Other Nuclei Processing Tasks - Involvement in most other projects which require nuclei processing - Single nuclei sequencing, and single nucleiATACsequencing Bacterial transformation and molecular cloning work -Golden Gate Cloning -Restriction Enzyme Cloning - Amplification of pDNA libraries -Preparation of all relevant media: Agar plates, Ampicillin working solution, LB Broth - Inventory of all relevant reagents -Plasmid Purification: Maxi Prep -Maintenance of all purified plasmids -Making glycerol stocks / storage -Coordinating cloning related work agendas with other members -Maintenance of cloning progress log Maintaining and storing current oligomer and primer pairs -record keeping of all incoming materials fromIDT -Preparation of 100uM and 10uM working solutions from stocks -Creating more working stocks of various concentrations if needed Coordinating withCUT&RUNcollaborators -Processing, sorting, and delivering nuclei samples to collaborator, etc. -GeneralPCR/qPCR work -Western Blot Work -Library Construction -Preparing/Sending samples for Sanger Sequencing, 10X, Bioanalyzer, Genewiz, and other collaborating Universities,NYGC -Shipping/ receiving domestic shipments P-card and Financial responsibilities -Ordering supplies for the lab -Reconciling purchases each month -Maintaining limit of p-card each month -Involvement in POs -Any other p-card related responsibilities Organizational Duties -Maintains extensive log of allATAC-seq related data -Maintains inventory log of oligomers and primers -Organized all protocols and shipping forms -Coordinating wet lab agendas with other lab members -Maintaining -20 C freezer organization -Ability to work independently Other duties/ responsibilities -Coordinate equipment delivery, installations, certifications and trainings. -Proactively mitigate laboratory issues including scheduling equipment repair for malfunctioning instruments and work orders for facilities management -Maintain cold storage (4/-20/-80/LN) organization ensuring all laboratory needs are met. - 4 C - -20 C -Maintain accurate logs of equipment use including usage by external laboratories. -ManageCTCNsupply inventory and prevent supplies shortages -Manage and maintain vendor-facing relationships with vendor representatives. -Provide technical training to new and rotating laboratory members for molecular biology: qpCR library quantification, library prep/pooling, nuclei processing, restriction enzyme cloning, golden gate cloning, maxi prep plasmid purification) -Distribute domestic shipments requiring a temperature-controlled supply chain. Create and distribute sample manifests, customs invoice, and other relevant documents to involved parties. -Manage laboratory hygiene ensuring TC waste collection, water bath, and fume hood are routinely cleaned. Specifically, TC related, cold storage related responsibilities (4/-20C) 10x responsibilities -Ensure laboratory personnel are in compliance with safety measures such as external member occupancy and social distancing guidelines. -Additional duties as necessary. -Create a consistent protocol for onboarding new lab members Bachelor's degree Hiring salary range $57,000 - $62,000 The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting. Columbia University is an Equal Opportunity Employer / Disability / Veteran